UTRN (англ. Utrophin) – білок, який кодується однойменним геном, розташованим у людей на короткому плечі 6-ї хромосоми. Довжина поліпептидного ланцюга білка становить 3 433 амінокислот, а молекулярна маса — 394 466.
Послідовність амінокислот
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MAKYGEHEAS | PDNGQNEFSD | IIKSRSDEHN | DVQKKTFTKW | INARFSKSGK | ||||
| PPINDMFTDL | KDGRKLLDLL | EGLTGTSLPK | ERGSTRVHAL | NNVNRVLQVL | ||||
| HQNNVELVNI | GGTDIVDGNH | KLTLGLLWSI | ILHWQVKDVM | KDVMSDLQQT | ||||
| NSEKILLSWV | RQTTRPYSQV | NVLNFTTSWT | DGLAFNAVLH | RHKPDLFSWD | ||||
| KVVKMSPIER | LEHAFSKAQT | YLGIEKLLDP | EDVAVQLPDK | KSIIMYLTSL | ||||
| FEVLPQQVTI | DAIREVETLP | RKYKKECEEE | AINIQSTAPE | EEHESPRAET | ||||
| PSTVTEVDMD | LDSYQIALEE | VLTWLLSAED | TFQEQDDISD | DVEEVKDQFA | ||||
| THEAFMMELT | AHQSSVGSVL | QAGNQLITQG | TLSDEEEFEI | QEQMTLLNAR | ||||
| WEALRVESMD | RQSRLHDVLM | ELQKKQLQQL | SAWLTLTEER | IQKMETCPLD | ||||
| DDVKSLQKLL | EEHKSLQSDL | EAEQVKVNSL | THMVVIVDEN | SGESATAILE | ||||
| DQLQKLGERW | TAVCRWTEER | WNRLQEINIL | WQELLEEQCL | LKAWLTEKEE | ||||
| ALNKVQTSNF | KDQKELSVSV | RRLAILKEDM | EMKRQTLDQL | SEIGQDVGQL | ||||
| LDNSKASKKI | NSDSEELTQR | WDSLVQRLED | SSNQVTQAVA | KLGMSQIPQK | ||||
| DLLETVRVRE | QAITKKSKQE | LPPPPPPKKR | QIHVDIEAKK | KFDAISAELL | ||||
| NWILKWKTAI | QTTEIKEYMK | MQDTSEMKKK | LKALEKEQRE | RIPRADELNQ | ||||
| TGQILVEQMG | KEGLPTEEIK | NVLEKVSSEW | KNVSQHLEDL | ERKIQLQEDI | ||||
| NAYFKQLDEL | EKVIKTKEEW | VKHTSISESS | RQSLPSLKDS | CQRELTNLLG | ||||
| LHPKIEMARA | SCSALMSQPS | APDFVQRGFD | SFLGRYQAVQ | EAVEDRQQHL | ||||
| ENELKGQPGH | AYLETLKTLK | DVLNDSENKA | QVSLNVLNDL | AKVEKALQEK | ||||
| KTLDEILENQ | KPALHKLAEE | TKALEKNVHP | DVEKLYKQEF | DDVQGKWNKL | ||||
| KVLVSKDLHL | LEEIALTLRA | FEADSTVIEK | WMDGVKDFLM | KQQAAQGDDA | ||||
| GLQRQLDQCS | AFVNEIETIE | SSLKNMKEIE | TNLRSGPVAG | IKTWVQTRLG | ||||
| DYQTQLEKLS | KEIATQKSRL | SESQEKAANL | KKDLAEMQEW | MTQAEEEYLE | ||||
| RDFEYKSPEE | LESAVEEMKR | AKEDVLQKEV | RVKILKDNIK | LLAAKVPSGG | ||||
| QELTSELNVV | LENYQLLCNR | IRGKCHTLEE | VWSCWIELLH | YLDLETTWLN | ||||
| TLEERMKSTE | VLPEKTDAVN | EALESLESVL | RHPADNRTQI | RELGQTLIDG | ||||
| GILDDIISEK | LEAFNSRYED | LSHLAESKQI | SLEKQLQVLR | ETDQMLQVLQ | ||||
| ESLGELDKQL | TTYLTDRIDA | FQVPQEAQKI | QAEISAHELT | LEELRRNMRS | ||||
| QPLTSPESRT | ARGGSQMDVL | QRKLREVSTK | FQLFQKPANF | EQRMLDCKRV | ||||
| LDGVKAELHV | LDVKDVDPDV | IQTHLDKCMK | LYKTLSEVKL | EVETVIKTGR | ||||
| HIVQKQQTDN | PKGMDEQLTS | LKVLYNDLGA | QVTEGKQDLE | RASQLARKMK | ||||
| KEAASLSEWL | SATETELVQK | STSEGLLGDL | DTEISWAKNV | LKDLEKRKAD | ||||
| LNTITESSAA | LQNLIEGSEP | ILEERLCVLN | AGWSRVRTWT | EDWCNTLMNH | ||||
| QNQLEIFDGN | VAHISTWLYQ | AEALLDEIEK | KPTSKQEEIV | KRLVSELDDA | ||||
| NLQVENVRDQ | ALILMNARGS | SSRELVEPKL | AELNRNFEKV | SQHIKSAKLL | ||||
| IAQEPLYQCL | VTTETFETGV | PFSDLEKLEN | DIENMLKFVE | KHLESSDEDE | ||||
| KMDEESAQIE | EVLQRGEEML | HQPMEDNKKE | KIRLQLLLLH | TRYNKIKAIP | ||||
| IQQRKMGQLA | SGIRSSLLPT | DYLVEINKIL | LCMDDVELSL | NVPELNTAIY | ||||
| EDFSFQEDSL | KNIKDQLDKL | GEQIAVIHEK | QPDVILEASG | PEAIQIRDTL | ||||
| TQLNAKWDRI | NRMYSDRKGC | FDRAMEEWRQ | FHCDLNDLTQ | WITEAEELLV | ||||
| DTCAPGGSLD | LEKARIHQQE | LEVGISSHQP | SFAALNRTGD | GIVQKLSQAD | ||||
| GSFLKEKLAG | LNQRWDAIVA | EVKDRQPRLK | GESKQVMKYR | HQLDEIICWL | ||||
| TKAEHAMQKR | STTELGENLQ | ELRDLTQEME | VHAEKLKWLN | RTELEMLSDK | ||||
| SLSLPERDKI | SESLRTVNMT | WNKICREVPT | TLKECIQEPS | SVSQTRIAAH | ||||
| PNVQKVVLVS | SASDIPVQSH | RTSEISIPAD | LDKTITELAD | WLVLIDQMLK | ||||
| SNIVTVGDVE | EINKTVSRMK | ITKADLEQRH | PQLDYVFTLA | QNLKNKASSS | ||||
| DMRTAITEKL | ERVKNQWDGT | QHGVELRQQQ | LEDMIIDSLQ | WDDHREETEE | ||||
| LMRKYEARLY | ILQQARRDPL | TKQISDNQIL | LQELGPGDGI | VMAFDNVLQK | ||||
| LLEEYGSDDT | RNVKETTEYL | KTSWINLKQS | IADRQNALEA | EWRTVQASRR | ||||
| DLENFLKWIQ | EAETTVNVLV | DASHRENALQ | DSILARELKQ | QMQDIQAEID | ||||
| AHNDIFKSID | GNRQKMVKAL | GNSEEATMLQ | HRLDDMNQRW | NDLKAKSASI | ||||
| RAHLEASAEK | WNRLLMSLEE | LIKWLNMKDE | ELKKQMPIGG | DVPALQLQYD | ||||
| HCKALRRELK | EKEYSVLNAV | DQARVFLADQ | PIEAPEEPRR | NLQSKTELTP | ||||
| EERAQKIAKA | MRKQSSEVKE | KWESLNAVTS | NWQKQVDKAL | EKLRDLQGAM | ||||
| DDLDADMKEA | ESVRNGWKPV | GDLLIDSLQD | HIEKIMAFRE | EIAPINFKVK | ||||
| TVNDLSSQLS | PLDLHPSLKM | SRQLDDLNMR | WKLLQVSVDD | RLKQLQEAHR | ||||
| DFGPSSQHFL | STSVQLPWQR | SISHNKVPYY | INHQTQTTCW | DHPKMTELFQ | ||||
| SLADLNNVRF | SAYRTAIKIR | RLQKALCLDL | LELSTTNEIF | KQHKLNQNDQ | ||||
| LLSVPDVINC | LTTTYDGLEQ | MHKDLVNVPL | CVDMCLNWLL | NVYDTGRTGK | ||||
| IRVQSLKIGL | MSLSKGLLEE | KYRYLFKEVA | GPTEMCDQRQ | LGLLLHDAIQ | ||||
| IPRQLGEVAA | FGGSNIEPSV | RSCFQQNNNK | PEISVKEFID | WMHLEPQSMV | ||||
| WLPVLHRVAA | AETAKHQAKC | NICKECPIVG | FRYRSLKHFN | YDVCQSCFFS | ||||
| GRTAKGHKLH | YPMVEYCIPT | TSGEDVRDFT | KVLKNKFRSK | KYFAKHPRLG | ||||
| YLPVQTVLEG | DNLETPITLI | SMWPEHYDPS | QSPQLFHDDT | HSRIEQYATR | ||||
| LAQMERTNGS | FLTDSSSTTG | SVEDEHALIQ | QYCQTLGGES | PVSQPQSPAQ | ||||
| ILKSVEREER | GELERIIADL | EEEQRNLQVE | YEQLKDQHLR | RGLPVGSPPE | ||||
| SIISPHHTSE | DSELIAEAKL | LRQHKGRLEA | RMQILEDHNK | QLESQLHRLR | ||||
| QLLEQPESDS | RINGVSPWAS | PQHSALSYSL | DPDASGPQFH | QAAGEDLLAP | ||||
| PHDTSTDLTE | VMEQIHSTFP | SCCPNVPSRP | QAM |
Кодований геном білок за функцією належить до фосфопротеїнів. Білок має сайт для зв'язування з молекулою актину, іонами металів, іоном цинку, іоном кальцію. Локалізований у клітинній мембрані, цитоплазмі, цитоскелеті, мембрані, клітинних контактах, синапсах.
Література
- Wilson J., Putt W., Jimenez C., Edwards Y.H. (1999). Up71 and up140, two novel transcripts of utrophin that are homologues of short forms of dystrophin.. Hum. Mol. Genet. 8: 1271 — 1278. PubMed DOI:10.1093/hmg/8.7.1271
- Burton E.A., Tinsley J.M., Holzfeind P.J., Rodrigues N.R., Davies K.E. (1999). A second promoter provides an alternative target for therapeutic up-regulation of utrophin in Duchenne muscular dystrophy.. Proc. Natl. Acad. Sci. U.S.A. 96: 14025 — 14030. PubMed DOI:10.1073/pnas.96.24.14025
- Ahn A.H., Kunkel L.M. (1995). Syntrophin binds to an alternatively spliced exon of dystrophin.. J. Cell Biol. 128: 363 — 371. PubMed DOI:10.1083/jcb.128.3.363
- Tommasi di Vignano A., Di Zenzo G., Sudol M., Cesareni G., Dente L. (2000). Contribution of the different modules in the utrophin carboxy-terminal region to the formation and regulation of the DAP complex.. FEBS Lett. 471: 229 — 234. PubMed DOI:10.1016/S0014-5793(00)01400-9
- Bhosle R.C., Michele D.E., Campbell K.P., Li Z., Robson R.M. (2006). Interactions of intermediate filament protein synemin with dystrophin and utrophin.. Biochem. Biophys. Res. Commun. 346: 768 — 777. PubMed DOI:10.1016/j.bbrc.2006.05.192
- Singh S.M., Bandi S., Winder S.J., Mallela K.M. (2014). The actin binding affinity of the utrophin tandem calponin-homology domain is primarily determined by its N-terminal domain.. Biochemistry 53: 1801 — 1809. PubMed DOI:10.1021/bi500149q
Див. також
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